qiagen 204143实时定量PCR试剂盒
qiagen 204143实时定量PCR试剂盒
QuantiTect SYBR Green PCR Kit使用SYBR Green I通过两步法real-time RT-PCR对cDNA目标序列进行高特异性的定量检测。热启动和*的实时定量PCR缓冲液确保了对cDNA目标序列高特异性和灵敏度的real-time定量检测。dNTP混合液含有dUTP,可选择性进行UNG处理。为便于使用,预混液可储存在2–8°C。
qiagen 204143实时定量PCR试剂盒
特异性定量检测。
在Rotor-Gene 3000上使用针对人BAX的QuantiTect Primer Assay和的试剂盒分析稀释的人白细胞cDNA。熔解曲线分析中出现单峰,NTC反应呈平直曲线,说明使用QuantiTect Kit和Assay可实现高特异性定量。NTC:无模板对照。Norm. Fluoro.:标准化的荧光。
at No./ID: 204141 QuantiTect SYBR Green PCR Kit (40) Trial kit for 40 x 50 µl reactions: 1 ml 2x QuantiTect SYBR Green PCR Master Mix, 2 ml RNase-Free Water |
Cat No./ID: 204143 QuantiTect SYBR Green PCR Kit (200) For 200x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 2 x 2 ml RNase-Free Water |
Cat No./ID: 204145 QuantiTect SYBR Green PCR Kit (1000) For 1000 x 50 µl reactions: 25 ml 2x QuantiTect SYBR Green PCR Master Mix, 20 ml RNase-Free Water |
Cat No./ID: 204163 QuantiTect SYBR Green PCR +UNG Kit (200) For 200 x 50 µl reactions: 3 x 1.7 ml 2x QuantiTect SYBR Green PCR Master Mix, 100 µl UNG Solution, 2 x 2 ml |