Qiagen凯杰981103 Strip Tubes and Caps 0.1ml
Qiagen凯杰981103 Strip Tubes and Caps 0.1ml
操作步骤:应用QIAGEN Plasmid Kits纯化过程中,通过离心取出细菌裂解液。然后将澄清的裂解液装入阴离子交换柱,在适当的低盐浓度和pH值条件下,阴离子交换柱会选择性地结合质粒DNA。RNA、蛋白质、代谢物和其他小分子杂质通过中盐浓度洗涤去除,应用高盐浓度缓冲夜洗脱超纯的质粒DNA(见流程图"Comparison of QIAGEN Plasmid Kit Procedures")。通过异丙醇沉淀浓缩并脱盐DNA,离心法收集DNA。
Qiagen凯杰981103 Strip Tubes and Caps 0.1ml,
IAGEN Plasmid Kits 纯化所得的质粒DNA非常适合于诸如转染(见图"转染效率 Vs. 质粒纯化方法")、克隆、手动或自动测序(包括毛细管测序)和体外转录等应用。
纯化PCR产物,100bp-10kb
高达95%的回收效率
三步骤回收DNA,片段zui大可达10 kb
上样液含有三色胶示踪染料,方便样本分析
Cat No./ID: 9018895 Rotor-Disc 100 Rotor Log in For holding Rotor-Disc 100 discs with reaction volumes of 15–25 µl in the Rotor-Gene Q; requires Rotor-Disc 100 Locking Ring |
Cat No./ID: 9018896 Rotor-Disc 100 Locking Ring Log in For locking a Rotor-Disc 100 in the Rotor-Disc 100 Rotor |
Cat No./ID: 9018897 Rotor-Disc Pipetting Aid Log in Aid for marking well during manual reaction setup on a Rotor-Disc Loading Block |
Cat No./ID: 9018898 Rotor-Disc Heat Sealer 110V Log in Heat sealing instrument for use with Rotor-Discs; requires Rotor-Disc 72 or 100 Loading Block |
Cat No./ID: 9018899 Rotor-Disc 72 Rotor Log in For holding Rotor-Disc 72 discs with reaction volumes of 20–25 µl in the Rotor-Gene Q; requires Rotor-Disc 72 Locking Ring |
Cat No./ID: 9018900 Rotor-Disc 72 Locking Ring Log in For locking a Rotor-Disc 72 in the Rotor-Disc 72 Rotor |
Cat No./ID: 9018901 Loading Block 72 x 0.1 ml Tubes Log in Aluminum block for manual reaction setup with a single-channel pipet in 72 x 0.1 ml tubes |
Cat No./ID: 9018902 Loading Block 72 x 0.1 ml Multi-channel Log in Aluminum block for reaction set-up with multichannel pipets in 72 x 0.1 ml tubes |
Cat No./ID: 9018903 72-Well Rotor Log in For holding Strip Tubes and Caps, 0.1 ml, with reaction volumes of 10–50 µl; requires Locking Ring 72-Well Rotor |
Cat No./ID: 9018904 Locking Ring 72-Well Rotor Log in For locking Strip Tubes and Caps, 0.1 ml in the 72-Well Rotor |
Cat No./ID: 9018905 Loading Block 96 x 0.2 ml Tubes Log in Aluminum block for manual reaction set-up in a standard 8 x 12 array using 96 x 0.2 ml tubes |
Cat No./ID: 9018906 36-Well Rotor Locking Ring Log in For locking PCR Tubes, 0.2 ml, in the 36-Well Rotor |
Cat No./ID: 9018907 36-Well Rotor Log in For holding PCR Tubes, 0.2 ml, with reaction volumes of 20–50 µl; requires 36-Well Rotor Locking Ring |
Cat No./ID: 9018908 Rotor Holder Log in Metal free-standing holder for assembling tubes and Rotor-Discs into rotors |
Cat No./ID: 9018910 Rotor-Disc 72 Loading Block Log in Aluminium block for manual and automated reaction setup in Rotor-Disc 72 discs |
Cat No./ID: 9019725 Rotor-Disc Heat Sealer 230V Log in Heat sealing instrument for use with Rotor-Discs; requires Rotor-Disc 72 or 100 Loading Block |
Cat No./ID: 9020286 Loading Block, Rotor-Disc 100 Easylift Ejector Inquire Ejector to be used in combination with Loading Block, Rotor-Disc 100 Easylift (cat. no. 9020287) |
Cat No./ID: 9020287 Loading Block, Rotor-Disc 100 Easylift Inquire Aluminium block for manual and automated reaction setup in Rotor-Disc 100 discs. The block covers 2 standard SBS positions in a vertical orientation on the QIAgility instrument worktable; ejection of the Rotor-Disc 100 is made convenient by using the Easylift ejector (cat. no. 9020286) |
Cat No./ID: 981005 PCR Tubes, 0.2 ml (1000) Log in 1000 thin-walled tubes for 1000 reactions of 20–50 µl |
Cat No./ID: 981008 PCR Tubes, 0.2 ml (10000) Log in 10 x 1000 thin-walled tubes for 10,000 reactions of 20–50 µl |
Cat No./ID: 981103 Strip Tubes and Caps, 0.1 ml (250) Log in 250 strips of 4 tubes and caps for 1000 reactions of 10–50 µl |
Cat No./ID: 981106 Strip Tubes and Caps, 0.1 ml (2500) Log in 10 x 250 strips of 4 tubes and caps for 10,000 reactions of 10–50 µl |
Cat No./ID: 981301 Rotor-Disc 72 (24) Log in 24 individually wrapped discs for 1728 reactions of 20–25 µl |
Cat No./ID: 981303 Rotor-Disc 72 (240) Log in 10 x 24 individually wrapped discs for 17,280 reactions of 20–25 µl |
Cat No./ID: 981311 Rotor-Disc 100 (30) Log in 30 individually wrapped discs for 3000 reactions of 15–25 µl |
Cat No./ID: 981313 Rotor-Disc 100 (300) Log in 10 x 30 individually wrapped discs for 30,000 reactions of 15–25 µl |
Cat No./ID: 981601 Rotor-Disc Heat Sealing Film (60) Log in 60 films for sealing Rotor-Disc 100 or Rotor-Disc 72 discs |
Cat No./ID: 981604 Rotor-Disc Heat Sealing Film (600) Log in 10 x 60 films for sealing Rotor-Disc 100 or Rotor-Disc 72 discs |
Rotor-Gene Q Accessories are for use with the Rotor-Gene Q thermal cycler.